Advisor(s)
Samuel John Gatley
Contributor(s)
Akio Ohta, Richard I. Duclos Jr
Date of Award
2009
Date Accepted
8-6-2009
Degree Grantor
Northeastern University
Degree Level
M.S.
Degree Name
Master of Science
Department or Academic Unit
Bouvé College of Health Sciences. Department of Pharmaceutical Sciences.
Keywords
Pharmaceutical science, Pharmacology, Health sciences
Subject Categories
Pharmaceutical technology
Disciplines
Pharmacy and Pharmaceutical Sciences
Abstract
We tried to obtain distribution maps of good quality for mouse brain sections for 14C after injecting [1-14C]acetate and [1-14C]acetaldehyde using vibratome/phosphorimager technique. Quantitative analysis of the images obtained with this technique showed significant differences in relative radioactivity between acetaldehyde and acetate in the hippocampus, with acetate levels higher than acetaldehyde. To confirm the results, microdissection experiments of mice brains were conducted, and radioactivity was quantified using liquid scintillation counting. The results were normalized to total brain radioactivity for each radiotracer and significantly higher hippocampus to whole brain ratio was confirmed for acetate compared to acetaldehyde. In contrast to the relative higher uptake of label in hippocampus after administration of acetate, the absolute brain uptake was higher for acetaldehyde in all regions.
Document Type
Master's Thesis
Rights Holder
Shachi R. Bhatt
Permanent URL
Recommended Citation
Bhatt, Shachi R., "Use of vibratome sectioning and phosphor Imaging to characterize distribution of 14C in brain after administration of radio-labeled acetaldehyde and acetate." (2009). Pharmaceutical Science Master's Theses. Paper 12. http://hdl.handle.net/2047/d10019479
Click button above to open, or right-click to save.
Additional Files
DefensePresentation.pptx (2814 kB)Thesis defense
Notes
Includes supplemental powerpoint file of thesis defense, 58 slides.