The transmission electron microscope (TEM) is extensively used in medical research because of its high magnification and resolution capabilities. One drawback of the TEM is that as the magnification increases, its field of view decreases, and more time is required to identify points of interest on a biological sample. This longer exposure can degrade the sample integrity. A solution is to first map the sample with fluorescent nanoparticles, flash freeze it in vitreous ice at cryogenic temperatures, then study the sample under the optical microscope (OM). The OM provides a much larger field of view, allowing the identification of the key points of interest, before transferring it to the TEM. The described solution is comprised of a custom optical microscope with a built-in cooling system that is compatible with current TEM equipment.
transmission electron microscope (TEM), optical microscope (OM)
Northeastern University, Department of Mechanical, Industrial and Manufacturing Engineering
Ali, Mohammad; Delcore, John; Kaufmann, Sarah; and Rezac, David, "Cryogenic Optical Microscope" (2007). Capstone Design Program: Mechanical Engineering. Paper 24. http://hdl.handle.net/2047/d10011390
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