Advisor(s)
Roger W. Giese
Abstract
With many researchers concentrating on a cure for cancer, there are some focusing on detecting genetic predispositions early enough for preventative measures. The Environmental Cancer Research Program (ERCP) is one such place. Specific research is being done on DNA adducts, however the bigger bulkier adducts are having low yields. This research focused on optimizing the conditions of the enzyme used to detach the DNA adducts from DNA. The data showed that that extended incubation of the enzyme at 45??C does not diminish the enzymatic rate, and increasing the concentration of Mg2+ in the buffer results in a lower enzymatic rate after extended incubation at 55 C.
Date Accepted
May 2007
Subject Categories
Cancer, Genetics
Keywords
DNA Adducts, Enzyme rate, Phosphodiesterase I
Degree Grantor
Northeastern University
Publication Date
5-3-2007
Permanent URL
Recommended Citation
Calhoun, Shawna, "Optimization of exonuclease digestion for isolation of DNA adducts" (2007). Honors Junior/Senior Projects. Paper 34. http://hdl.handle.net/2047/d10006564