Abstract

Mitochondria are dynamic organelles capable of undergoing fission and fusion. Overexpression of a dominant negative mutant of a mitochondrial fission protein (DLP1-K38A) inhibits total mitochondrial fission resulting in tubular mitochondria. In this work, we present a quantitative analysis of mitochondrial tubulation using FARSIGHT, a multidimensional image analysis tool. It has been found that in a control cell, total mitochondrial volume is distributed into many isolated mitochondrial elements. In contrast, it is seen that tubular mitochondria caused by overexpression of DLPI-K38A contains 80% of the mitochondrial volume within a single mitochondrial element.

Notes

Poster presented at 2007 Thrust R2D Image Understanding and Sensor Fusion Methods Conference

Keywords

mitochondria, mitochondrial fission protein, FARSIGHT

Subject Categories

Mitochondrial pathology

Disciplines

Biomedical Engineering and Bioengineering

Publisher

Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems (Gordon-CenSSIS)

Publication Date

2007

Rights Holder

Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems (Gordon-CenSSIS)

Click button above to open, or right-click to save.

Share

COinS