Abstract
Mitochondria are dynamic organelles capable of undergoing fission and fusion. Overexpression of a dominant negative mutant of a mitochondrial fission protein (DLP1-K38A) inhibits total mitochondrial fission resulting in tubular mitochondria. In this work, we present a quantitative analysis of mitochondrial tubulation using FARSIGHT, a multidimensional image analysis tool. It has been found that in a control cell, total mitochondrial volume is distributed into many isolated mitochondrial elements. In contrast, it is seen that tubular mitochondria caused by overexpression of DLPI-K38A contains 80% of the mitochondrial volume within a single mitochondrial element.
Keywords
mitochondria, mitochondrial fission protein, FARSIGHT
Subject Categories
Mitochondrial pathology
Disciplines
Biomedical Engineering and Bioengineering
Publisher
Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems (Gordon-CenSSIS)
Publication Date
2007
Rights Holder
Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems (Gordon-CenSSIS)
Permanent URL
Recommended Citation
Dwarakapurm, Saritha; Roysam, Badrinath; Lin, Gang; and Mitra, Kasturi, "Quantitative Analysis of Mitochondrial Tubulation Using 3D Imaging" (2007). Research Thrust R2 Presentations. Paper 35. http://hdl.handle.net/2047/d10009000
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COinS
Notes
Poster presented at 2007 Thrust R2D Image Understanding and Sensor Fusion Methods Conference