Reflectance confocal microscopy is a powerful in-vivo modality for imaging superficial layers of biological tissue, especially for human skin. Three dimensional imaging capability enables confocal microscopy to resolve structures of upper skin layer cells. However, sub-celluar structure and corresponding functional organelles play more important parts in skin diseases diagnosis and monitoring. We present a new multi-spectral reflectance confocal microscopy to achieve sub-celluar functional imaging in skin by utilizing our unique Keck multi-modality microscope. Spectral information and a modified Mie scattering model are incorporated to identify distribution of melanin and mitochondria in cells. Ex-vivo and phantom experimental results are presented. Further development of this new modality may lead to future clinical applications.


Poster presented at the 2007 R2C Multi Spectral Discrimination Methods Conference


Reflectance Confocal Microscopy, Skin, in-vivo

Subject Categories

Three-dimensional imaging, Diagnostic imaging, Cancer--Diagnosis


Biomedical Engineering and Bioengineering


Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems (Gordon-CenSSIS)

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Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems (Gordon-CenSSIS)

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