Abstract

Oocyte (egg) morphology has been shown to correlate to viability. By observing the localization of subsurface organelles we hope to learn more about oocyte structure. Mouse oocytes were collected from superovulated female C57BL/6 mice using a hormone dosing regimen. Live oocytes were then stained using multiple organellespecific fluorescent dyes and imaged on the Keck 3D Fusion Microscope (3DFM) to highlight different components of the eggs. The dyes used stained chromosomes, mitochondria, endoplasmic reticulum, membrane, tubulin, and lysosomes. Images were collected using epifluorescence and Differential Interference Contrast (DIC) microscopy and were compiled and overlaid using Metamorph software. These images serve as useful tools in exhibiting organization of developing oocytes.

Notes

Poster presented at the 2007 Validating TestBED and Research on Real World Problems for the I-PLUS Development Conference

Keywords

oocytes, 3DFM, differential interference contrast (DIC) microscopy

Subject Categories

Three-dimensional imaging

Disciplines

Biomedical Engineering and Bioengineering

Publisher

Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems (Gordon-CenSSIS)

Publication Date

2007

Rights Holder

Bernard M. Gordon Center for Subsurface Sensing and Imaging Systems (Gordon-CenSSIS)


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