The development of a submicron optical fiber "supertip" has provided advantages over previously produced submicron tips, such as facilitating insertion of these sensors into cells while minimizing damage to the cell membrane. Fiber optic ion correlation-based nanosensors for sodium, potassium and chloride employing these "supertips" have been applied to the monitoring of ion concentrations in single mouse oocytes. These sensors have also been used to monitor the effect of an ion channel-blocking agent. In order to address the challenge associated with single-cell simultaneous measurement of multiple analytes, the use of submicron optical fiber multiprobes has been explored.
optodes, chemical sensors, fiber optic, multiprobe, intracellular measurements
Optodes, Optical detectors, Chemical detectors
Bioimaging and biomedical optics
Copyright 1998 One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Society of Photo-Optical Instrumentation Engineers
Kopelman, Raoul; Miller, Michael T.; Brasuel, Murphy; Clark, Heather; Hoyer, Marion; and Philbert, Martin, "Optochemical nanosensors for intracellular chemical measurement" (1998). Bouvé Faculty Publications. Paper 6. http://hdl.handle.net/2047/d20000325
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