A novel optical biosensor matrix has been developed to exploit the native fluorescence of certain proteins. This matrix uses a gold colloid monolayer attached to an end of a fiber as a substrate for protein attachment. The effect of the gold monolayer size has been investigated through the techniques of fluorescence, scanning electron microscopy, and transmission electron microscopy. It has been shown that the size ofthe gold colloid does produce a marked difference in the fluorescence intesity measured. It is surmised through the use of microscopy techniques that the intensity changes seen in the fluorescence emission are not a result of surface coverage, or availability of sites for protein adsorption, but instead of quenching or enhancement by the gold itself.
optodes, gold colloids, biosensor, sensor
Optodes, Biosensors, Collodial gold.
Bioimaging and biomedical optics
Copyright 2000 One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Society of Photo-Optical Instrumentation Engineers
Clark, Heather; Merritt, Greg; and Kopelman, Raoul, "Novel optical biosensors using a gold colloid monolayer substrate" (2000). Bouvé Faculty Publications. Paper 3. http://hdl.handle.net/2047/d20000322
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