Abstract

We examined and compared a variety of existing protocols for enumeration of bacteria from marine sandy sediments. The focus was on how to dislodge bacteria from sediment particles; a commercial blender, an ultrasonic cleaner, and an ultrasonic cell disrupter were tested. The ultrasonic cell disrupter was found to be the most efficient device for bacterial dislodgment. With a 5 mm microtip vibrating at 109 μm amplitude and 20 kHz, the optimal sonication time of small (≤ 0.5 cm³) samples was 180 s. Having identified the optimal dislodgment treatment, we went through other steps of sediment bacteria enumeration (use of surfactants, thoroughness of sample washing, bacterial staining and counterstaining, etc.), striving for additional ways of improvement. The result was a protocol that, in our study, gave bacterial counts superior to those obtained by any other protocol currently in use.

Notes

Originally published in Marine Ecology Progress Series 117 (1995): 289-298. http://www.int-res.com/abstracts/meps/v117/

Keywords

bacteria, benthos, enumeration, fluorescence, ultrasonic treatment

Subject Categories

Marine bacteria - Counting

Disciplines

Bacteriology | Marine Biology

Publisher

Inter-Research

Publication Date

2-9-1995

Rights Information

Copyright 1995

Rights Holder

Inter-Research

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